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A simple method for the isolation of murine peripheral blood lymphocytes
Journal of Immunological Methods
|January 1, 1978
Abstract:
A simple, rapid and reproducible semi-microtechnique was developed for the isolation of murine peripheral blood lymphocytes. Murine peripheral blood was collected from brachial vessels and centrifuged through a Ficoll--Hypaque gradient in a microfuge tube yielding 1--1.5 X 10(6) lymphocytes per mouse.