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Related Experiment Videos

Drug interference in clinical chemistry: studies on ascorbic acid.

G Siest, W Appel, G B Blijenberg

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |February 1, 1978
    PubMed
    Summary

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    Ascorbic acid (vitamin C) can interfere with clinical chemistry tests. It significantly impacts glucose, uric acid, and creatinine analysis, potentially leading to inaccurate results at therapeutic doses.

    Area of Science:

    • Clinical Chemistry
    • Analytical Toxicology
    • Biochemistry

    Background:

    • Ascorbic acid (vitamin C) is a common supplement with potential to affect laboratory test results.
    • Clinical laboratories must be aware of drug interferences to ensure accurate patient diagnosis.
    • The European Communities' Bureau of Reference established an expert group to investigate drug interferences.

    Purpose of the Study:

    • To evaluate the interference of therapeutic and elevated doses of ascorbic acid on common clinical chemistry methods.
    • To identify specific analytes affected by ascorbic acid and quantify the interference.
    • To eliminate confounding variables like methodology and reagents to isolate ascorbic acid's effects.

    Main Methods:

    • A bipartite study was jointly planned, executed, and evaluated.

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  • Local, personal, and methodological variations were minimized.
  • Ascorbic acid's impact was assessed on various clinical chemistry analytes using different reaction types, kits, and apparatus.

    • Main Results:

    • No significant interference was observed for urea, cholesterol, calcium, protein, bilirubin, aspartate aminotransferase, and alkaline phosphatase.
    • Ascorbic acid at therapeutic concentrations distinctly interfered with the analysis of glucose, uric acid, creatinine, and inorganic phosphate.
    • The extent and direction of interference varied based on the specific analytical method, kit, and equipment used, sometimes yielding unusable values.

    Conclusions:

    • Ascorbic acid interferes with several key clinical chemistry assays, particularly glucose, uric acid, and creatinine.
    • The interference is dose-dependent and method-specific, highlighting the need for careful interpretation of results in patients taking ascorbic acid supplements.
    • Clinicians and laboratory professionals should be aware of these interferences to avoid misdiagnosis and ensure reliable patient care.