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Age and susceptibility of Swiss mice for mouse adenovirus, strain FL
Abstract:
NMRI mice of different ages (24 hours, 10 to 12 days old, adults) were inoculated intraperitoneally with varying doses of mouse adenovirus. The susceptibility to disease was high in newborn mice (1 LD50 equalled 7 TCID50, as measured in mouse kidney cell cultures), medium in 10 days' old mice and almost nil in adult animals. The survival time was not greatly influenced by the virus dose. The susceptibility to infection is greatest in adults and lowest in 10 day old mice; these show a susceptibility equal to kidney cell cultures. In newborn mice, the virus spreads via the blood to all organs tested except for the intestinal tract, peak titers being reached in kidney and liver for at least 52 days, together with a sustained viruria. Adult mice could be infected by oral or intranasal installation.
Insights
Newborn mice are highly susceptible to mouse adenovirus, while adult mice show increased susceptibility to infection. This study details age-related differences in adenovirus disease and infection in NMRI mice.
Area of Science:
- Virology
- Immunology
- Mouse Models
Background:
- Mouse adenovirus is a significant pathogen in laboratory mice.
- Understanding age-related susceptibility is crucial for disease control and experimental design.
Purpose of the Study:
- To investigate the impact of age on mouse adenovirus susceptibility and disease progression.
- To characterize the viral spread and shedding patterns in mice of different age groups.
Main Methods:
- NMRI mice of varying ages (newborn, 10-12 days old, adult) were inoculated with mouse adenovirus.
- Viral susceptibility was assessed by Lethal Dose 50 (LD50) and Tissue Culture Infectious Dose 50 (TCID50) in mouse kidney cell cultures.
- Viral spread, organ distribution, and viruria were monitored in infected mice.
Main Results:
- Newborn mice exhibited high susceptibility to disease (1 LD50 = 7 TCID50), with widespread viral dissemination to organs except the intestinal tract.
- Adult mice demonstrated the highest susceptibility to infection, despite lower disease severity compared to newborns.
- Mice aged 10-12 days showed intermediate susceptibility, comparable to kidney cell cultures. Sustained viruria was observed in newborn mice for at least 52 days.
Conclusions:
- Age is a critical factor influencing mouse adenovirus susceptibility and disease outcome.
- Newborn mice are most vulnerable to severe disease, while adults are more prone to infection, suggesting complex immune responses.
- The findings highlight the importance of considering age in mouse adenovirus research and management.