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Acute cytomegalovirus glomerulonephritis: an experimental model
Laboratory Investigation; a Journal of Technical Methods and Pathology
|September 1, 1980
Summary
Murine cytomegalovirus infection causes temporary glomerulonephritis in mice. Viral transport via the glomerulus, juxtaglomerular zone, and distal tubule may lead to persistent kidney lesions.
Area of Science:
- Nephrology
- Virology
- Immunology
Background:
- Murine cytomegalovirus (MCMV) infection in HA/ICR mice leads to acute glomerulonephritis.
- The condition is characterized by intranuclear inclusions in mesangial cells.
Purpose of the Study:
- To investigate the pathogenesis of MCMV-induced glomerulonephritis.
- To elucidate the route of virus transport leading to viruria and potential long-term renal damage.
Main Methods:
- Intraperitoneal injection of mice with an LD(10) of MCMV.
- Light microscopy, electron microscopy, and immunofluorescence techniques were employed.
- Analysis of viral antigen distribution and renal pathology over time.
Main Results:
- Acute glomerulonephritis with mesangial cell inclusions was observed.
- Viruria and proteinuria peaked on day 3 and resolved by day 10.
- Electron microscopy revealed virus accumulation in intercellular channels within the mesangium and juxtaglomerular area.
- Viral antigen was cleared from glomeruli but persisted in the juxtaglomerular zone in 40% of mice, leading to lesions.
Conclusions:
- The glomerular mesangium, juxtaglomerular zone, and distal convoluted tubule are involved in MCMV transport and viruria.
- Persistent viral antigen in this pathway can cause juxtaglomerular lesions and interstitial nephritis.