Restriction of herpes simplex virus by Ama 1 cells. An analysis of viral macromolecule synthesis

Ama 1 cells, and alpha-amanitin-resistant subline of CHO cells, restricted herpes simplex virus-1 and -2 replication. The infection was characterized by i) induction of typical cytopathology; ii) appearance of all the major virus proteins, glycoproteins and DNA earlier than in HEp-2 cells, followed by shut off of virus macromolecule synthesis; iii) defective maturation of viral particles, i.e. scarce assembly and lack of envelopment. The early shut off of viral DNA and protein synthesis, and the altered glycoprotein pattern may account for herpes simplex virus restriction.