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[Bordetella pertussis bacteriophage].

I A Lapaeva, S M Mebel', N A Pereverzev

Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii

|May 1, 1980

Summary

Researchers isolated the Bordetella pertussis bacteriophage, revealing a previously unknown phage system within the Bordetella genus. This phage effectively lyses Bordetella parapertussis strains and can alter Bordetella pertussis virulence.

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Area of Science:

Microbiology
Virology
Bacteriology

Background:

Bordetella pertussis is a significant human pathogen.
The existence of bacteriophages within the Bordetella genus was previously unknown.
Understanding phage-host interactions is crucial for microbial research.

Purpose of the Study:

To isolate and characterize bacteriophages of Bordetella pertussis.
To investigate the potential of Bordetella phages for inter-species lysis.
To explore the characteristics of lysogenic Bordetella pertussis variants.

Main Methods:

Isolation of Bordetella pertussis bacteriophage.
Confirmation using electron microscopy and agar layer titration.
Activation of lysogenic strains with mitomycin C.
Testing phage activity against Bordetella parapertussis strains.
Characterization of lysogenic variants' phenotypic properties.

Main Results:

Bordetella pertussis bacteriophage was successfully isolated and confirmed.
The phage demonstrated broad lytic activity against all tested Bordetella parapertussis strains.
Lysogenic Bordetella pertussis variants were obtained, showing increased toxin content and virulence.
Phage system within the Bordetella genus was revealed for the first time.

Conclusions:

A novel Bordetella pertussis bacteriophage and its lytic capabilities have been identified.
This discovery opens avenues for understanding Bordetella phage-host dynamics.
The characterized phage and lysogenic variants have implications for Bordetella research and potential therapeutic strategies.

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