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Related Experiment Videos

Insertion element IS102 resides in plasmid pSC101.

H Ohtsubo, M Zenilman, E Ohtsubo

    Journal of Bacteriology
    |October 1, 1980
    PubMed
    Summary
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    Researchers discovered a novel 1-kilobase insertion sequence, IS102, during plasmid recombination studies. This sequence duplicated at recombination junctions and showed structural similarities to known mobile genetic elements.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Plasmid recombination is a key mechanism for genetic exchange in bacteria.
    • Understanding the molecular basis of recombination is crucial for microbial genetics.
    • Previous studies suggested that insertion sequences can mediate plasmid recombination.

    Purpose of the Study:

    • To investigate the mechanism of in vivo recombination between plasmid pHS1 and ColE1.
    • To characterize the structure and properties of recombinant plasmids.
    • To identify and define novel insertion sequences involved in recombination.

    Main Methods:

    • In vivo selection for tetracycline resistance at restrictive temperature.
    • Restriction endonuclease digestion and electron microscopy heteroduplex analysis.

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  • Nucleotide sequence analysis of parental and recombinant plasmids.

    • Main Results:

    • In vivo recombination occurred between pHS1 and ColE1, forming recombinant plasmids.
    • A unique 1-kilobase sequence from pHS1 was duplicated at the junctions of integrated pHS1 within ColE1.
    • This 1-kilobase sequence was identified as a novel insertion sequence, IS102, with terminal inverted repeats and similarities to Tn903.
    • Recombination was RecA-independent.

    Conclusions:

    • The 1-kilobase sequence, IS102, is a novel insertion sequence mediating plasmid recombination.
    • IS102 exhibits characteristics of mobile genetic elements, including duplication and terminal repeats.
    • The findings contribute to the understanding of plasmid evolution and genetic diversity in bacteria.