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Expression of a cell surface glycoprotein (p180) related to cell-substratum adhesion during differentiation of mouse
Abstract:
Mouse myeloid leukemia M1 cells were induced to differentiate in vitro into macrophages and granulocytes by various inducers including ascitic fluid. Differentiated M1 cells induced with ascitic fluid expressed a differentiation-associated cell surface glycoprotein with a molecular weight of 180,000 (p180), which can be labeled by lactoperoxidase-catalyzed radioiodination or metabolic labeling with L-[14C]fucose. p180 was also induced by treatment with conditioned medium of hamster embryo cells, dexamethasone, dibutyryl cyclic adenosine 3':5'-monophosphate, and prostaglandin E1. Ascitic fluid, conditioned medium of hamster embryo cells, and dexamethasone induced all the differentiation-associated properties tested, whereas dibutyryl cyclic adenosine 3':5'-monophosphate and prostaglandin E1 induced lysozyme activity and adhesiveness to the substratum but not phagocytosis, locomotive activity, Fc receptors, or morphological changes. The adherent cells induced by dibutyryl cyclic adenosine 3':5'-monophosphate produced a large amount of p180, while the floating cells produced very little, but no difference was detected in the lysozyme activities of the two cell types. These results suggest that p180 is associated with cell-substratum adhesion of differentiated M1 cells.
Insights
Mouse myeloid leukemia M1 cells differentiated into macrophages and granulocytes, expressing a surface glycoprotein (p180). This protein is linked to cell adhesion, particularly in adherent cells induced by specific agents.
Area of Science:
- Cell Biology
- Biochemistry
- Developmental Biology
Background:
- Mouse myeloid leukemia M1 cells are a model for studying myeloid differentiation.
- Cell surface glycoproteins play crucial roles in cell adhesion and differentiation.
- Understanding differentiation pathways can provide insights into cellular functions.
Purpose of the Study:
- To investigate the differentiation of M1 cells induced by various agents.
- To identify and characterize differentiation-associated cell surface molecules.
- To explore the functional significance of these molecules in M1 cell differentiation.
Main Methods:
- In vitro induction of M1 cell differentiation using ascitic fluid and other agents.
- Cell surface labeling via lactoperoxidase-catalyzed radioiodination and metabolic labeling with L-[14C]fucose.
- Analysis of differentiation-associated properties including lysozyme activity, phagocytosis, and cell morphology.
Main Results:
- Ascitic fluid, conditioned medium, and dexamethasone induced comprehensive M1 cell differentiation.
- Dibutyryl cyclic adenosine 3':5'-monophosphate and prostaglandin E1 induced partial differentiation, including lysozyme activity and adhesion.
- A differentiation-associated glycoprotein (p180) was identified and found to be highly expressed in adherent M1 cells.
Conclusions:
- The glycoprotein p180 is induced during M1 cell differentiation.
- p180 expression is strongly associated with cell-substratum adhesion in differentiated M1 cells.
- Specific inducers trigger distinct differentiation pathways and molecular responses in M1 cells.