Benzo[a]pyrene metabolism in cells productively infected with simian virus 40

The metabolism of benzo[a]pyrene (BP) by cell cultures and cell-free extracts of the monkey kidney cell line CV-1 was studied in uninfected and Simian virus 40 (SV40) infected cells. Metabolites formed were separated by high-pressure liquid chromatography (HPLC) and quantified by liquid scintillation techniques. The profiles metabolites formed by uninfected and SV40 infected cells were similar except that SV40 infected cell cultures metabolized BP at an increased rate relative to uninfected cells. In addition, SV40 infected cell cultures and cell-free extracts produced an unknown compound which eluted between the 3-hydroxybenzo[a]pyrene and BP fractions. This material does not have a retention time characteristic of any of the known metabolites of BP. Labelled BP and/or its metabolites were bound to the viral DNA and histone components of intracellular viral minichromosomes as well as the viral DNA and proteins of mature virions.