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Factors which disorganize microtubules or microfilaments increase the frequency of cell transformation by polyoma
Abstract:
Griseofulvin, 12-O-tetradecanoyl phorbol-13-acetate, melittin, epidermal growth factor, vinblastine, cytochalasin B, podophyllotoxin, colcemid, and colchicine were unable to transform cells but could increase from 8- to 40-fold the frequency of cell transformation by polyoma virus. The 3T3-like cells were resting at confluence and were exposed to the drug only during the 1st week after viral infection. Griseofulvin, a tumor promoter, reduced or increased the frequency of transformation depending on the dose with which the infected cells were treated. The antitumor activity of tumor promoters is discussed.
Insights
Several compounds, including tumor promoters, enhanced polyoma virus-induced cell transformation. Griseofulvin
Area of Science:
- Cell Biology
- Virology
- Oncology
Background:
- Cell transformation is a critical step in cancer development.
- Polyoma virus is a model system for studying cell transformation.
- Tumor promoters can influence the process of carcinogenesis.
Purpose of the Study:
- To investigate the effect of various compounds on polyoma virus-mediated cell transformation.
- To explore the dose-dependent effects of the tumor promoter griseofulvin on cell transformation frequency.
- To discuss the antitumor activity of tumor promoters.
Main Methods:
- 3T3-like cells were infected with polyoma virus.
- Cells were treated with various compounds, including griseofulvin, during the first week post-infection.
- The frequency of cell transformation was quantified.
Main Results:
- Griseofulvin, 12-O-tetradecanoyl phorbol-13-acetate, melittin, epidermal growth factor, vinblastine, cytochalasin B, podophyllotoxin, colcemid, and colchicine did not induce cell transformation independently.
- These compounds increased the frequency of polyoma virus-induced cell transformation by 8- to 40-fold.
- Griseofulvin exhibited a dose-dependent effect, either reducing or increasing transformation frequency.
Conclusions:
- Certain compounds can act as potent enhancers of viral cell transformation.
- Griseofulvin's effect on transformation is concentration-dependent.
- The study provides insights into the complex interplay between viral oncogenesis and tumor promoters.