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Residual pathologic changes and 99mtechnetium pyrophosphate uptake following coxsackievirus B3 perimyocarditis in
Abstract:
The myocardial uptake of technetium-99m pyrophosphate (99mTc-PYP) in perimyocarditis induced by coxsackievirus B3 in BALB/c mice was studied on 3rd--90th day after the inoculation. 99mTc-PYP uptake ratio, measured by the ratio of cpm/gm for the heart to cpm/gm for the skull, began to increase five days after virus inoculation and reached a maximum on the seventh day. After the 14th day, 99mTc-PYP ratio began to decrease, however, on the 90th day, a high 99mTc-PYP uptake was shown in mice with severe perimyocardial fibrosis and calcification. The present findings may provide a futher basis upon which 99mTc-PYP imaging may be applied to viral perimyocarditis in humans.
Insights
Technetium-99m pyrophosphate (99mTc-PYP) uptake in the heart increases during viral perimyocarditis in mice, peaking early but remaining high with fibrosis. This suggests 99mTc-PYP imaging may aid human diagnosis.
Area of Science:
- Cardiology
- Virology
- Nuclear Medicine
Background:
- Viral perimyocarditis, often caused by coxsackievirus B3, can lead to significant cardiac complications.
- Technetium-99m pyrophosphate (99mTc-PYP) scintigraphy is used to detect myocardial damage.
Purpose of the Study:
- To investigate the myocardial uptake of 99mTc-PYP in a murine model of coxsackievirus B3-induced perimyocarditis over 90 days.
- To evaluate the correlation between 99mTc-PYP uptake and the progression of myocardial fibrosis and calcification.
Main Methods:
- BALB/c mice were inoculated with coxsackievirus B3 to induce perimyocarditis.
- Myocardial uptake of 99mTc-PYP was measured using the ratio of counts per minute per gram (cpm/gm) in the heart to the skull at various time points (3-90 days post-inoculation).
Main Results:
- 99mTc-PYP uptake ratio increased significantly by day 5, peaking on day 7 post-inoculation.
- While the uptake ratio decreased after day 14, it remained elevated on day 90 in mice with severe perimyocardial fibrosis and calcification.
Conclusions:
- 99mTc-PYP uptake dynamics reflect the acute and chronic phases of viral perimyocarditis in mice.
- These findings support the potential utility of 99mTc-PYP imaging for diagnosing viral perimyocarditis in humans, particularly in identifying chronic fibrotic changes.