Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polycistronic vesicular stomatitis virus RNA transcripts.

R C Herman, M Schubert, J D Keene

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Vorticity-Crystalline Order Coupling in Supersolids: Excitations and Reentrant Phases.

    Physical review letters·2026
    Same author

    Quantum Carpets of Higgs Quasiparticles in a Supersolid.

    Physical review letters·2025
    Same author

    One-hundred-fifteen consecutive bladder exstrophies successfully closed in a single nationally commissioned centre.

    Journal of pediatric urology·2025
    Same author

    Does intra-epidermal electrical stimulation activate mechano- and thermo-nociceptors? A discrimination approach.

    Journal of neuroscience methods·2025
    Same author

    ICG mapping of postoperative lymphatic leakage in the groin: a video article and literature review.

    Facts, views & vision in ObGyn·2024
    Same author

    Are changes in nociceptive withdrawal reflex magnitude a viable central sensitization proxy? Implications of a replication attempt.

    Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology·2022
    Same journal

    Costunolide ameliorates autoimmune uveitis by targeting USP15 to suppress TNF-α-induced retinal endothelial inflammation.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    A ligandable PNT domain establishes ERG as a directly targetable oncogenic driver in prostate cancer.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Identification of cellular intermediates unveils unique enzymes for flagellar glycan biosynthesis in <i>Clostridioides difficile</i>.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    The structure of correlated variability reflects task-relevant information in sensory neurons.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Shared neurogenetic substrates of nonplanning impulsivity and procrastination.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    HIV-1 capsid interactions with Nuclear Pore Complex components support nuclear entry via affinity gradient.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Researchers describe a method to isolate RNA sequences from vesicular stomatitis virus (VSV). This technique reveals precise transcription mechanisms, showing how VSV RNA polymerase links genetic information after polyadenylation.

    Area of Science:

    • Molecular Biology
    • Virology
    • RNA Transcription

    Background:

    • Polycistronic RNAs are synthesized in vitro by vesicular stomatitis virus (VSV).
    • Understanding the precise mechanisms of RNA processing and transcription in viruses is crucial for molecular biology and virology.
    • The junction sequences between linked messages in polycistronic RNAs hold key information about transcription termination and reinitiation.

    Purpose of the Study:

    • To develop and describe a procedure for enriching specific RNA sequences.
    • To analyze the sequences at the junction of linked messages in VSV polycistronic RNAs.
    • To elucidate the mechanism of transcription and polyadenylation in VSV.

    Main Methods:

    • Development of a procedure to enrich for junctional sequences in polycistronic RNAs.

    Related Experiment Videos

  • In vitro synthesis of polycistronic RNAs using vesicular stomatitis virus (VSV).
  • Sequence analysis of the enriched RNA fragments.

    • Main Results:

    • Identified precise transcripts at the junction of linked messages.
    • These sequences contain the intercistronic dinucleotide and a specific pentanucleotide (5'--C-U-G-U-U--3').
    • The identified sequences were covalently linked to the 3'-side of the intervening poly(A) sequence.

    Conclusions:

    • The VSV transcriptase performs polyadenylation of mRNAs.
    • The transcriptase can resume direct and precise transcription of the genome.
    • This process occurs without reinitiation or skipping of nucleotides, indicating a highly accurate transcription mechanism.