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Updated: Aug 13, 2026

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Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
Published on: July 23, 2017
Purification of phytase from rat intestinal mucosa
Abstract:
Phytase has been purified from intestinal mucosa of rats fed on a phytic acid-enriched diet. The purification consisted of four steps: homogenate preparation, ultrafiltration procedure, DEAE cellulose chromatography, and Sephadex G-100 chromatography. The enzyme was purified 828-fold, as can be seen from the polyacrylamide gel electrophoresis pattern.

