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Polypeptide changes in Sendai virus-infected cells.

R Repanovici, O Mihalache, R Iliescu

    Virologie
    |July 1, 1980
    PubMed
    Summary
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    Researchers identified all structural and nonstructural Sendai virus proteins in infected cells using SDS-PAGE. Specific structural proteins (NP, P, M) were found bound to ribosomes and could be washed off.

    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Sendai virus is a paramyxovirus that infects various cell types.
    • Understanding viral protein synthesis and localization is crucial for comprehending viral replication and pathogenesis.

    Purpose of the Study:

    • To investigate the appearance and localization of virus-specific proteins in Sendai virus-infected cells.
    • To identify which viral proteins associate with cellular ribosomes.

    Main Methods:

    • High-resolution SDS-polyacrylamide gel electrophoresis (SDS-PAGE) was employed.
    • Analysis of total cell lysates and ribosome fractions from infected chick embryo fibroblasts and chorioallantoic membrane cells.

    Main Results:

    • All structural Sendai virus polypeptides and the nonstructural polypeptide B were detected in total infected cell lysates.

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  • Only structural polypeptides NP, P, and M were identified in the ribosome fraction.
  • These ribosome-associated polypeptides were removable by washing with 1 M NH4Cl.

    • Conclusions:

    • Sendai virus infection leads to the synthesis of both structural and nonstructural viral proteins within host cells.
    • Specific structural viral proteins (NP, P, M) exhibit association with cellular ribosomes during infection.
    • The association of these proteins with ribosomes is likely a surface interaction, as indicated by their removal through washing.