Related Experiment Video
Updated: May 11, 2026
10:21
Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
Published on: October 7, 2018
Summary
Researchers created SV40 recombinants with a mouse alpha-globin gene. Both viruses produced functional globin mRNA in monkey cells, indicating the gene
Area of Science:
- Molecular Biology
- Gene Expression
- Virology
Background:
- SV40 recombinants are valuable tools for studying gene expression.
- Understanding globin gene regulation is crucial for hemoglobin research.
Purpose of the Study:
- To construct SV40 recombinants containing a mouse alpha-globin gene.
- To investigate the functionality of the globin gene promoter in vivo.
- To assess globin mRNA synthesis and processing in infected cells.
Main Methods:
- Construction of two SV40 recombinants with a mouse alpha-globin gene.
- Infection of monkey kidney cells with recombinant viruses.
- Analysis of globin mRNA synthesis and 5' terminus identification.
Main Results:
- Both SV40 recombinants directed the synthesis of functional mouse alpha-globin mRNA.
- The synthesized globin mRNA exhibited a 5' terminus identical to authentic globin mRNA.
- Efficient globin expression was observed, dependent on the promoter's origin.
Conclusions:
- The cloned mouse alpha-globin gene contains a functional promoter.
- This promoter is accurately recognized and utilized in monkey kidney cells.
- SV40 recombinants serve as effective vectors for studying globin gene expression.
More Related Videos
Related Concept Videos
01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
02:11Reporter Genes
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Commonly used reporter...