Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Leukotriene A. Isolation from human polymorphonuclear leukocytes.

O Rådmark, C Malmsten, B Samuelsson

    The Journal of Biological Chemistry
    |December 25, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Slow-reacting substances and leukotrienes.

    Immunology today·2014
    Same author

    Microsomal prostaglandin E synthase-1 and 5-lipoxygenase: potential drug targets in cancer.

    Journal of internal medicine·2010
    Same author

    Disposition of CP-671, 305, a selective phosphodiesterase 4 inhibitor in preclinical species.

    Xenobiotica; the fate of foreign compounds in biological systems·2005
    Same author

    Attenuation of the virulence of Porphyromonas gingivalis by using a specific synthetic Kgp protease inhibitor.

    Infection and immunity·2002
    Same author

    Expression of microsomal glutathione S-transferase type 3 mRNA in the rat nervous system.

    Neuroscience·2002
    Same author

    Resistance profiles of cyclic and linear inhibitors of HIV-1 protease.

    Antiviral chemistry & chemotherapy·2002

    Researchers isolated leukotriene A (LTA), an arachidonic acid metabolite, from human cells. A new method efficiently extracts and esterifies this unstable intermediate for further study.

    Area of Science:

    • Biochemistry
    • Immunology

    Background:

    • Leukotriene A (LTA) is a transient intermediate in arachidonic acid metabolism.
    • LTA is crucial for synthesizing stable leukotrienes, potent inflammatory mediators.
    • Its inherent instability, particularly acid hydrolysis, complicates isolation and analysis.

    Purpose of the Study:

    • To develop a reliable method for the isolation and characterization of leukotriene A.
    • To overcome the challenges posed by LTA's chemical instability.
    • To enable further research into LTA's role in biological processes.

    Main Methods:

    • Isolation of LTA from human polymorphonuclear leukocytes.
    • Esterification of LTA to its methyl ester using diazomethane in an alkaline aqueous phase.
    • Extraction of the methyl ester into an aprotic solvent.

    Related Experiment Videos

  • Characterization using chromatographic and chemical property comparison.

    • Main Results:

    • Successfully isolated and esterified leukotriene A as its methyl ester.
    • Developed a method to prevent rapid hydrolysis of the allylic epoxide intermediate.
    • Confirmed the identity of the isolated compound with synthetic methyl ester of 5 (S)-trans-5,6-oxido-7,9-trans-11,14-cis-eicostatetraenoic acid via comparative analysis.

    Conclusions:

    • A robust method for LTA methyl ester isolation and extraction has been established.
    • This method facilitates the study of LTA, an important but unstable intermediate.
    • The findings enable further investigation into leukotriene biosynthesis and function.