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Updated: Jul 18, 2026

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
09:40

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Published on: September 23, 2011

Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent

B Errede, T S Cardillo, F Sherman

    Cell
    |November 1, 1980
    PubMed

    Insights

    ROAM mutations in yeast cause gene overproduction, especially in haploid strains. This overproduction is linked to conjugation ability and involves Ty element insertions near genes.

    Area of Science:

    • Molecular biology
    • Yeast genetics

    Background:

    • ROAM mutations in Saccharomyces cerevisiae lead to overproduction of gene products.
    • The extent of overproduction varies between different yeast mating types and ploidy levels.

    Purpose of the Study:

    • To investigate the genetic basis and regulatory mechanisms underlying ROAM mutations in yeast.
    • To determine the role of Ty elements in ROAM-mediated gene expression.

    Main Methods:

    • Genetic analysis of ROAM mutants in Saccharomyces cerevisiae.
    • Cloning and characterization of the ROAM mutant gene CYC7-H2.
    • Investigating the effect of mutations affecting conjugation (ste mutations) on ROAM expression.

    Main Results:

    • Overproduction is higher in haploid strains capable of conjugation compared to non-conjugating diploids.
    • Diploid strains capable of mating show overproduction, regardless of sporulation ability.
    • Insertion of a Ty1 element in the 5' noncoding region of CYC7 is identified in a ROAM mutant.
    • The ste7 mutation, which prevents conjugation, reduces ROAM overproduction.

    Conclusions:

    • ROAM mutations are associated with the insertion of Ty elements, likely responsible for altered gene expression.
    • ROAM mutations appear to respond to signals regulating yeast conjugation.
    • Ty element sequences may act as receptors for conjugation-related regulatory signals.

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