A modified enzymatic technique for production of cell suspensions of a murine fibrosarcoma

Collagenase, elastase, and mild mechanical procedures were used to obtain numerous viable single cells in suspension from a murine fibrosarcoma of C57BL/6 origin. This technique produced suspensions with minimal debris. The suspensions were more than 90% viable and yielded over 1.25 x 10(8) cells/g of solid tumor.