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Related Experiment Videos

The ral gene of phage lambda. III. Interference with E. coli ATP dependent functions.

L Debrouwere, M Van Montagu, J Schell

    Molecular & General Genetics : MGG
    |January 1, 1980
    PubMed
    Summary

    The phage lambda ral gene counteracts ATP-dependent E. coli functions, including RecBC recombination and host restriction. Ral may act as a second anti-termination factor, antagonizing the transcription termination factor rho.

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    Area of Science:

    • Molecular Biology
    • Bacteriology
    • Genetics

    Background:

    • The phage lambda ral gene is known to overcome host-controlled restriction and enhance DNA modification in Escherichia coli.
    • Previous studies established ral's role in phage lambda's interaction with its host.

    Purpose of the Study:

    • To investigate the broader functions of the ral gene beyond host restriction and modification.
    • To elucidate the molecular mechanisms by which ral influences E. coli cellular processes.

    Main Methods:

    • Assessing the impact of ral expression on E. coli recombination pathways, specifically the RecBC pathway.
    • Analyzing the phenotypes of E. coli strains with constitutive ral expression.
    • Comparing ral-mediated effects with known phenotypes of mutations in the transcription termination factor rho.

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  • Investigating the interplay between ral, general recombination, and host-controlled restriction in rho-deficient strains.

    • Main Results:

    • Ral plays a minor role in phage lambda's lytic cycle but significantly impacts other ATP-dependent E. coli functions.
    • Ral specifically reduces the efficiency of RecBC-mediated recombination.
    • Constitutive expression of ral in E. coli leads to phenotypes similar to those observed in rho-mutant strains.
    • Reduced general recombination and host-controlled restriction were noted in rho-deficient E. coli strains.

    Conclusions:

    • The findings suggest ral functions as an anti-termination factor, distinct from the lambda N gene product.
    • Ral appears to directly antagonize the activity of the transcription termination factor rho.
    • Ral's multifaceted role extends to influencing recombination and potentially transcription termination in E. coli.