Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Synaptic localization of kainic acid binding sites.

A C Foster, E E Mena, D T Monaghan

    Nature
    |January 1, 1981
    PubMed
    Summary

    Kainic acid (KA), a potent neuronal excitant, targets distinct receptors separate from glutamate. This study reveals KA binding sites are enriched in synaptic junctions, explaining its neurotoxic effects in specific brain regions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Parenteral penicillin apparatus.

    The Pharmaceutical journal·2010
    Same author

    Central infusion of the melanocortin receptor antagonist agouti-related peptide (AgRP(83-132)) prevents cachexia-related symptoms induced by radiation and colon-26 tumors in mice.

    Peptides·2007
    Same author

    Identification of differential melanocortin 4 receptor agonist profiles at natively expressed receptors in rat cortical astrocytes and recombinantly expressed receptors in human embryonic kidney cells.

    Neuropharmacology·2006
    Same author

    A photometric redshift of z = 6.39 +/- 0.12 for GRB 050904.

    Nature·2006
    Same author

    Central administration of peptide and small molecule MC4 receptor antagonists induce hyperphagia in mice and attenuate cytokine-induced anorexia.

    Peptides·2005
    Same author

    Microinjection of urocortin 2 into the dorsal raphe nucleus activates serotonergic neurons and increases extracellular serotonin in the basolateral amygdala.

    Neuroscience·2004

    Area of Science:

    • Neuroscience
    • Neuropharmacology
    • Cell Biology

    Background:

    • Kainic acid (KA) is a potent neuronal excitant with complex effects on the central nervous system (CNS).
    • Its neurotoxic effects resemble conditions like Huntington's chorea and status epilepticus.
    • KA's mechanism of action is distinct from classical glutamate receptors, acting on a unique receptor class.

    Purpose of the Study:

    • To investigate the distribution of kainic acid (KA) binding sites in the rat brain.
    • To elucidate the relationship between KA receptor localization and its neurotoxic effects.
    • To understand the role of junctional versus extrajunctional receptors in KA's CNS action.

    Main Methods:

    • Isolation of synaptic junctions from rat brain.
    • In vitro autoradiography to map KA binding sites.
    • Correlation of binding site distribution with known neurotoxic areas.

    Main Results:

    • KA binding sites are significantly enriched in isolated synaptic junctions.
    • Autoradiography demonstrates concentration of binding sites in specific terminal fields.
    • These enriched areas correspond to regions where KA exhibits potent neurotoxicity.

    Conclusions:

    • KA binding sites are predominantly located at synaptic junctions.
    • The enrichment of KA receptors at synapses explains its selective neurotoxic action.
    • This finding provides crucial insights into KA's mechanism of action in the CNS.

    Related Experiment Videos