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Related Experiment Videos

[Phosphonate esterase activity in human serum (author's transl)].

M Labadie, P M Laplaud, G Lachatre

    Pathologie-Biologie
    |February 1, 1980
    PubMed
    Summary

    A new spectrophotometric assay measures phosphonate esterase activity in human serum. Elevated levels are linked to pregnancy, estrogenic drugs, and prostate neoplasms.

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    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Enzyme Assays

    Background:

    • Phosphonate esterase activity in human serum is not well-characterized.
    • Existing methods for enzyme activity measurement may lack simplicity or specificity.

    Purpose of the Study:

    • To develop and validate a simple spectrophotometric assay for human serum phosphonate esterase activity.
    • To establish reference values and investigate factors influencing serum phosphonate esterase levels.

    Main Methods:

    • Spectrophotometric assay using p-nitrophenol and phenyl-phosphonic acid ester.
    • Incubation at 30°C.
    • Analysis of human serum samples.

    Main Results:

    • The assay demonstrated good reproducibility.
    • Mean serum phosphonate esterase values were determined.
    • Activity was significantly increased during pregnancy and with estrogenic drug administration.

    Conclusions:

    • A reliable spectrophotometric method for serum phosphonate esterase assay is established.
    • Pregnancy and estrogenic drugs are associated with increased serum phosphonate esterase activity.
    • This assay may be useful in clinical diagnostics related to pregnancy, hormonal therapies, and prostate conditions.

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