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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
Published on: March 31, 2016
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Differentiation of porcine enterovirus serotypes by complement fixation
Research in Veterinary Science
|July 1, 1980
Summary
A new microtitre plate complement fixation test effectively differentiates eleven porcine enterovirus serotypes and swine vesicular disease virus. This method accurately identified 71 field isolates, aiding in porcine enterovirus strain differentiation.
Area of Science:
- Veterinary Virology
- Immunology
- Molecular Diagnostics
Background:
- Porcine enteroviruses (PEVs) are significant pathogens in swine.
- Accurate serotyping is crucial for disease control and epidemiological studies.
- Existing methods for PEV serotyping can be laborious and time-consuming.
Purpose of the Study:
- To develop and validate a sensitive and specific microtitre plate complement fixation test (CFT) for differentiating PEV serotypes.
- To compare the diagnostic performance of the developed CFT against known serotypes and field isolates.
Main Methods:
- Development of a microtitre plate-based complement fixation test.
- Utilized guinea pig antisera raised against inactivated, purified virus antigens.
- Tested eleven distinct PEV serotypes and swine vesicular disease virus (SVDV).
- Evaluated 71 additional PEV strains and isolates for serotype identification.
Main Results:
- The microtitre plate CFT clearly distinguished between the eleven PEV serotypes and SVDV.
- High specificity was demonstrated, with no cross-reactivity observed between the tested serotypes.
- All 71 porcine enterovirus field strains and isolates were successfully and accurately identified to one of the eleven serotypes.
Conclusions:
- The developed microtitre plate CFT is a reliable and efficient tool for the serological differentiation of porcine enteroviruses.
- This assay facilitates accurate identification of PEV serotypes in diagnostic and epidemiological investigations.
- The method offers a standardized approach for porcine enterovirus typing, aiding in disease management strategies.
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