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Genetic variability in Halobacterium halobium.

F Pfeifer, G Weidinger, W Goebel

    Journal of Bacteriology
    |January 1, 1981
    PubMed
    Summary
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    Halobacterium halobium shows high spontaneous mutation rates, often linked to insertions in the pHH1 plasmid. These genetic changes affect gas vacuole formation and purple membrane synthesis.

    Area of Science:

    • Microbiology
    • Genetics
    • Molecular Biology

    Background:

    • Halobacterium halobium displays remarkable genetic instability.
    • Spontaneous mutations frequently alter key cellular functions.

    Purpose of the Study:

    • Investigate the genetic basis of spontaneous mutations in Halobacterium halobium.
    • Determine the role of the pHH1 plasmid in observed phenotypic variations.

    Main Methods:

    • Analysis of spontaneous mutants for altered phenotypes (gas vacuoles, purple membrane).
    • Genomic analysis to identify mutations, particularly insertions in the pHH1 plasmid.
    • Comparison of vac, rub, and pum mutants with their wild-type counterparts.

    Main Results:

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  • Mutations affecting gas vacuole formation (vac) occur at a high frequency (10^-2).
  • Mutations affecting bacterioruberin (Rub) or purple membrane (Pum) synthesis occur at lower frequencies (10^-4).
  • Most mutants, except ret mutants, exhibit insertions, rearrangements, or deletions in the pHH1 plasmid, suggesting its central role in variability.

    • Conclusions:

    • Plasmid genes on pHH1 likely control gas vacuole formation in Halobacterium halobium.
    • The pHH1 plasmid is crucial for maintaining genetic stability and is involved in regulating various phenotypes.