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Restriction and modification in Bacillus subtilis: identification of a gene in the temperate phage SP beta coding for
Molecular & General Genetics : MGG
|January 1, 1980
Abstract:
A gene coding for a modifying DNA-methyltransferase which methylates the central C in the BsuR recognition sequence 5'GGCC was identified in the genome of the temperature Bacillus subtilis phage SP beta. This gene is expressed only after induction of the prophage by either mitomycin C or UV. The presence of active methyltransferase in induced cells leads to modification of BsuR recognition sites in SP beta DNA as well as in heterologous DNA.