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Updated: Oct 9, 2026

Purification of Mitochondria from Yeast Cells
Published on: August 24, 2009
[Changes in phospholipid composition of mitochondria after freezing-warming in the presence of Na2S204]
Abstract:
The phospholipid composition of mitochondria membranes subjected to rapid freezing (300-400 degrees C per 1 min) to -196 degrees C and subsequent slow warning (at 20 degrees C) was determined by the method of chromatography in the thin layer of silicagel. Under such conditions of freezing and warming a significant decrease in lecithin and ethanolamine phosphatide content is observed in the mitochondria membranes. When freezing the suspension of mitochondria in the medium containing Na2S2O4 in a concentration of 0.05 M only the lipid component changes slightly.
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