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[In vivo and in vitro activity of Streptococcus faecium extract on Herpes simplex virus]
Abstract:
The effects of substance or substance extracted from Str. faecium SF 68 on HSV-1 are evaluated. The "in vivo" assay show that bacterial extract introduced i.p. in mice simultaneously with HSV-1 brought about 100% of survival, but bacterial extract after virus challenge brought about complete mortality of mice. "In vitro" assays show that bacterial extract reduce significantly PFU number. It seemed that Str. faecium extract affected the virus at the stage of adsorption on the host cells.
Insights
A bacterial extract from *Str. faecium* SF 68 demonstrated protective effects against HSV-1 in mice when administered simultaneously with the virus. However, delayed administration led to complete mortality, suggesting a critical timing for its antiviral activity.
Area of Science:
- Microbiology
- Virology
- Immunology
Context:
- Herpes Simplex Virus type 1 (HSV-1) is a common human pathogen.
- Bacterial extracts are being explored for potential therapeutic applications.
- Understanding the interaction between bacterial components and viral infections is crucial.
Purpose:
- To evaluate the antiviral effects of a *Str. faecium* SF 68 extract against HSV-1.
- To determine the efficacy of the extract in both prophylactic and therapeutic settings.
- To investigate the mechanism of action of the extract on HSV-1.
Summary:
- In vivo studies showed 100% survival in mice when the bacterial extract was administered intraperitoneally simultaneously with HSV-1.
- Conversely, administration of the extract after viral challenge resulted in complete mortality.
- In vitro assays indicated a significant reduction in plaque-forming units (PFU) and suggested that the extract interferes with viral adsorption to host cells.
Impact:
- This research highlights the potential of *Str. faecium* SF 68 extract as a prophylactic agent against HSV-1.
- The findings emphasize the importance of administration timing for the extract's efficacy.
- Further investigation into the extract's antiviral mechanisms could lead to novel therapeutic strategies.