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Immunoreactive collagenase and bone resorption.

C Francois-Gillet, J M Delaissé, Y Eeckhout

    Biochimica Et Biophysica Acta
    |February 18, 1981
    PubMed
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    Inactive collagenase forms can be detected using specific antibodies. Mouse bone resorption may involve enzymes other than collagenase, as its accumulation in culture is heparin-dependent and not linked to parathyroid hormone.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • Collagenase plays a role in bone matrix resorption.
    • Understanding collagenase activity and regulation is crucial for bone biology.

    Purpose of the Study:

    • To investigate methods for detecting inactive collagenase forms.
    • To elucidate the role of collagenase in mouse bone resorption.

    Main Methods:

    • Antibody inhibition assays using various inactive collagenase forms.
    • Analysis of immunoreactive collagenase accumulation in mouse bone cultures.

    Main Results:

    • Inactive collagenase forms (procollagenase, complexes, degraded enzyme) were detected via antibody exclusion.
    • Immunoreactive collagenase accumulated in bone cultures only with heparin.

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  • Collagenase accumulation did not correlate with parathyroid hormone-induced bone resorption.

    • Conclusions:

    • A method was developed to detect enzymatically inactive collagenase.
    • Evidence suggests collagenase is not the primary enzyme system responsible for mouse bone matrix resorption.
    • Heparin influences collagenase accumulation independently of parathyroid hormone-mediated bone resorption.