Calcium dependence of synaptic transmission in the hippocampal slice

'Population' afferent spike and 'population' EPSP were recorded with extracellular microelectrodes in slices of hippocampal tissue maintained in vitro. Calcium concentration was changed in the bathing solution, and calcium activity ([Ca2+]0) was measured in interstitial fluid of the slice with ion-selective microelectrodes. Synaptic transfer was a non-linear continuous function of [Ca2+]0. Deviation of [Ca2+]0 by 0.1 mM from the 1.2 mM control level caused a change of approximately 15% in the slope of the input-output function.