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Transmembrane potential changes associated with superoxide release from human granulocytes.

G S Jones, K VanDyke, V Castranova

    Journal of Cellular Physiology
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

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    Stimulants like PMA trigger superoxide release in human granulocytes by altering membrane potential. Depolarization signals the initiation of the respiratory burst in these phagocytes.

    Area of Science:

    • Immunology
    • Cellular Physiology

    Background:

    • Human granulocytes are key immune cells involved in pathogen defense.
    • The respiratory burst, a critical function of phagocytes, involves superoxide anion release.
    • Understanding the signaling pathways that initiate the respiratory burst is crucial for immune response research.

    Purpose of the Study:

    • To investigate the role of transmembrane potential shifts in initiating the respiratory burst in human granulocytes.
    • To differentiate the effects of various stimulants on granulocyte membrane potential and superoxide release.

    Main Methods:

    • Human granulocytes were treated with stimulants: concanavalin A, phorbol myristate acetate (PMA), N-formyl-methionyl-leucyl-phenylalanine (FMLP), and A23187.
    • Membrane potential shifts were monitored using the fluorescent probe Di-S-C3(5).

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  • Superoxide anion release and chemiluminescence were measured.

    • Main Results:

    • Concanavalin A, PMA, and FMLP induced a biphasic shift in membrane potential (depolarization followed by hyperpolarization).
    • A23187 caused a monophasic, prolonged depolarization.
    • Depolarization was dependent on external sodium and calcium, while hyperpolarization was inhibited by ouabain.
    • These membrane potential changes preceded superoxide secretion.

    Conclusions:

    • Membrane depolarization acts as a critical signal initiating the respiratory burst in human granulocytes.
    • Different signaling pathways are involved in the response to various stimulants.
    • The findings provide insights into the early events of phagocyte activation.