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Fast stopped-flow microcalorimeter.

B Balko, P Bowen, R L Berger

    Journal of Biochemical and Biophysical Methods
    |January 1, 1981
    PubMed
    Summary
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    This study introduces a fast stopped-flow microcalorimeter for precise reaction kinetics. The instrument achieves 3 ms time resolution and corrects for heat loss, enabling accurate thermodynamic measurements.

    Area of Science:

    • Chemical Kinetics
    • Biophysical Chemistry
    • Analytical Chemistry

    Background:

    • Accurate measurement of reaction kinetics and thermodynamics is crucial in chemistry and biology.
    • Existing microcalorimetry techniques may have limitations in speed and sensitivity.

    Purpose of the Study:

    • To describe a novel fast stopped-flow microcalorimeter.
    • To validate its performance for kinetic and thermodynamic measurements.

    Main Methods:

    • Utilized a stopped-flow technique with a thermistor and AC bridge for rapid measurements.
    • Employed computer finite element simulation techniques (FEST) for data correction.
    • Tested with known reactions: NaOH/HCl, NaHCO3/HCl, and glyclyglycine/CO2.

    Main Results:

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    • Achieved a time resolution of 3 ms for half-life measurements.
    • Demonstrated a sensitivity of 100 mu degree C.
    • Reported experimental error less than 2% compared to published values.
    • Microcalorimeter is adiabatic to within 2% for 2 s.

    Conclusions:

    • The developed stopped-flow microcalorimeter is a reliable tool for fast kinetic and thermodynamic studies.
    • FEST effectively corrects for instrumental limitations, enhancing accuracy.
    • The instrument is suitable for reactions requiring small sample volumes (0.3 ml).