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Plasminogen activator is an apparent lymphocyte mitogen.

S D Cohen, E Israel, B Spiess-Meier

Journal of Immunology (Baltimore, Md. : 1950)

|April 1, 1981

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Avian sarcoma virus-transformed cells release plasminogen activator (PA), a substance that stimulates lymphocyte proliferation. Inhibiting PA blocks this mitogenic effect, confirming PA

Area of Science:

Immunology
Virology
Biochemistry

Background:

Avian sarcoma virus (ASV)-transformed cells exhibit distinct properties compared to normal cells.
Culture fluids from transformed cells can induce mitogenic responses in lymphocytes.

Purpose of the Study:

To investigate the role of plasminogen activator (PA) in the mitogenic effect observed in lymphocytes exposed to ASV-transformed cell culture fluids.
To determine if PA acts as a lymphocyte mitogen.

Main Methods:

Correlating PA activity in culture medium with lymphocyte mitogenic capacity.
Assessing the impact of agents that modulate PA levels (phorbol myristate acetate, dexamethasone) on mitogenesis.
Evaluating the effect of specific PA inhibitors (benzamidine, diisopropylfluorophosphate) and non-inhibitors (epsilon-amino-caproic acid, trasylol) on lymphocyte responsiveness.

Related Experiment Videos

Testing the mitogenic potential of purified urokinase, an enzyme with similar substrate specificity to PA.

Main Results:

PA activity in culture fluids positively correlated with lymphocyte mitogenic capacity.
Phorbol myristate acetate treatment increased both PA levels and mitogenesis.
Dexamethasone treatment reduced lymphocyte mitogenic activity by inhibiting PA.
Specific PA inhibitors blocked lymphocyte proliferation, while other enzyme inhibitors did not.
Purified urokinase demonstrated lymphocyte stimulatory activity.

Conclusions:

Plasminogen activator (PA) is strongly suggested to function as a lymphocyte mitogen.
PA released by ASV-transformed cells plays a key role in inducing lymphocyte proliferation.