Does glial uptake affect GABA responses? AN intracellular study on rat dorsal root ganglion neurones in vitro

1. Using single barrel pipettes, intracellular records were obtained from surface neurones of isolated rat dorsal root ganglia (DRG) impaled under microscopic vision.2. Responses to gamma-aminobutyric acid (GABA) were elicited either by ionophoresis or by placing drops of concentrated GABA solutions directly into the flow of superfusing Ringer. Using this latter method it was estimated that the GABA concentration eliciting threshold ( approximately 1 mV) responses was 3-20 muM.3. Short (