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Updated: Jul 24, 2026
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
Published on: March 10, 2017
A new approach for assaying LH by using a target cell response
Abstract:
Replicate cultures of 50000 mouse Leydig cells were used to obtain linear log dose-response curves to LH. This hormone augmented the activity of an endogenous alkaline phosphatase, which was subsequently allowed to act on p-nitrophenyl phosphate, the coloured end-point being measured with a spectrophotometer. The linear portion of the curve extended from 0.2 to 3.2 mi.u. LH/ml.
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