Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Basement membrane antigen in Wilms' tumor.

W A Franklin, J C Ringus

    Laboratory Investigation; a Journal of Technical Methods and Pathology
    |April 1, 1981
    PubMed
    Summary

    Researchers identified a basement membrane antigen in Wilms' tumors, suggesting these kidney cancers produce their own basement membrane components. This finding offers new insights into tumor biology and potential therapeutic targets.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A rare missense mutation in CHRNA4 associates with smoking behavior and its consequences.

    Molecular psychiatry·2016
    Same author

    Mer or Axl receptor tyrosine kinase inhibition promotes apoptosis, blocks growth and enhances chemosensitivity of human non-small cell lung cancer.

    Oncogene·2012
    Same author

    Insulin-like growth factor receptor 1 (IGF1R) expression and survival in surgically resected non-small-cell lung cancer (NSCLC) patients.

    Annals of oncology : official journal of the European Society for Medical Oncology·2009
    Same author

    EGFR regulation by microRNA in lung cancer: correlation with clinical response and survival to gefitinib and EGFR expression in cell lines.

    Annals of oncology : official journal of the European Society for Medical Oncology·2008
    Same author

    EGFR FISH assay predicts for response to cetuximab in chemotherapy refractory colorectal cancer patients.

    Annals of oncology : official journal of the European Society for Medical Oncology·2007
    Same author

    Predicting gene promoter methylation in non-small-cell lung cancer by evaluating sputum and serum.

    British journal of cancer·2007

    Area of Science:

    • Nephrology
    • Oncology
    • Biochemistry

    Background:

    • Wilms' tumor is a pediatric kidney cancer.
    • The origin of basement membrane components in Wilms' tumor is not fully understood.

    Purpose of the Study:

    • To prepare and characterize a basement membrane antigen.
    • To investigate the presence and localization of this antigen in Wilms' tumors.

    Main Methods:

    • Preparation of basement membrane antigen via reduction and alkylation.
    • Partial purification using gel filtration chromatography.
    • Immunohistochemical analysis using indirect immunofluorescence.

    Main Results:

    • A basement membrane antigen was successfully prepared and purified.
    • The antigen was detected within five Wilms' tumors, adjacent to tumor cells.
    • Ultrastructural observations showed similar flocculent electron-dense material.

    Conclusions:

    • The study provides immunohistochemical evidence for Wilms' tumors producing basement membrane components.
    • This suggests autocrine production of extracellular matrix by tumor cells.
    • Further research into these components may reveal novel therapeutic strategies.

    Related Experiment Videos