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Paramagnetic changes in pulmonary tumors.

W Lohmann, K G Bensch, J Schreiber

    Zeitschrift Fur Naturforschung. Section C, Biosciences
    |January 1, 1981
    PubMed
    Summary
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    Electron spin resonance (ESR) reveals distinct free radical differences in human lung tissue. Tumors show increased semidehydroascorbate (SDA) radicals, while within tumors, concentrations decrease, indicating altered ascorbic acid metabolism.

    Area of Science:

    • Biochemistry
    • Medical Physics
    • Oncology

    Background:

    • Free radicals play a role in cellular metabolism and disease.
    • Ascorbic acid (Vitamin C) is a key antioxidant involved in cellular processes.
    • Electron spin resonance (ESR) is a technique sensitive to free radicals.

    Purpose of the Study:

    • To investigate differences in free radical concentration and spectral characteristics between healthy and tumorous human lung tissues.
    • To explore the role of semidehydroascorbate (SDA) radicals in lung cancer.
    • To understand the impact of ascorbic acid levels on free radical signals in lung samples.

    Main Methods:

    • Electron spin resonance (ESR) spectroscopy was applied to healthy and tumorous human lung samples.
    • Analysis focused on free radical concentration and spectral shapes.

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  • Experiments included the addition of ascorbic acid and oxidizing agents (H2O2) to assess their effects.

    • Main Results:

    • Healthy lung tissue showed a less prominent SDA radical signal due to high oxygen levels.
    • Tumorous lung tissue exhibited increased spin concentration and a more pronounced SDA peak, suggesting altered ascorbic acid interactions.
    • Within tumors, spin concentration was significantly reduced, likely due to elevated ascorbic acid levels.
    • Ascorbic acid addition enhanced these observed effects, while H2O2 reversed them.

    Conclusions:

    • ESR can detect significant differences in free radical profiles between healthy and cancerous lung tissues.
    • Changes in SDA radical signals correlate with tumor formation and altered ascorbic acid metabolism.
    • These findings highlight the potential of ESR in studying oxidative stress and metabolic changes in lung cancer.