A new site-specific endonuclease StuI from Streptomyces tubercidicus

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Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
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Published on: March 16, 2012

H Shimotsu, H Takahashi, H Saito

    Gene
    |November 1, 1980
    PubMed

    Abstract:

    A new sequence-specific endonuclease, StuI, produced by Streptomyces tubercidicus KCC S-0054, was identified and partially purified. StuI recognizes the hexanucleotide "palindromic" sequence (Formula: see text), and cleaves it at the middle, producing blunt ends.

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