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Updated: Oct 7, 2026
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Published on: June 5, 2015
Nucleotide sequence of a cloned cDNA corresponding to secreted mu chain of mouse immunoglobulin
Abstract:
The cDNA complementary to a mouse immunoglobulin mu heavy chain mRNA has been cloned into the PstI site of the plasmid vector pBR322. A hybrid plasmid pmu 183.5 containing a 1850 bp insert has been selected by differential screening. The nucleotide sequence of the inset encodes the four constant domains, the terminal piece and the 3'-untranslated region.
Insights
Researchers cloned mouse immunoglobulin mu heavy chain complementary DNA (cDNA) into a plasmid vector. This clone, pmu 183.5, contains the nucleotide sequence encoding key parts of the mu heavy chain, aiding further study.
Area of Science:
- Molecular Biology
- Immunogenetics
- Recombinant DNA Technology
Background:
- Immunoglobulin mu heavy chain is crucial for B cell development and antibody production.
- Understanding the genetic basis of immunoglobulin heavy chains is vital for immunology research.
Purpose of the Study:
- To clone and characterize the complementary DNA (cDNA) encoding the mouse immunoglobulin mu heavy chain.
- To facilitate further studies on immunoglobulin gene expression and function.
Main Methods:
- Complementary DNA (cDNA) synthesis from mouse immunoglobulin mu heavy chain mRNA.
- Cloning of cDNA into the PstI site of the pBR322 plasmid vector.
- Selection of hybrid plasmid (pmu 183.5) using differential screening.
- Nucleotide sequencing of the cloned insert.
Main Results:
- Successfully cloned a 1850 bp cDNA insert encoding the mouse immunoglobulin mu heavy chain.
- The insert contains sequences for the four constant domains, terminal piece, and 3'-untranslated region.
- The hybrid plasmid pmu 183.5 was isolated and characterized.
Conclusions:
- The cloned cDNA provides a valuable tool for studying mouse immunoglobulin mu heavy chain structure and regulation.
- This research contributes to the understanding of antibody gene expression at the molecular level.
