Complex formation of spin-labeled 9-aminoacridine with DNA and polynucleotides

Complex formation between spin-labeled 9-aminoacridine and DNA or polynucleotides has been studied by differential spectrophotometry and ESR. The differential spectra of the strong type 9-aminoacridine-DNA complex showed characteristic absorption bands at 270 and 290 nm, and the intensity ratio of these bands varied according to the degree of DNA denaturation. The ESR spectra of this complex were characterized by slow rotation of the radical; as the macromolecule became increasingly denatured and in the polynucleotide complex, a rapid signal appeared in the ESR spectrum. The temperature at which DNA undergoes a structural transition in the premelting region could be determined from the temperature dependence of the ESR spectral form of the dye-DNA complex. The spectral characteristics of the complexes give additional information about structural disturbances in DNA.