A solid phase radioimmunoassay for poliovirus D-antigen

A solid phase radioimmunoassay has been developed for the determination of poliovirus D-antigen over the range 1-100 D-units/ml. A dilution series of a D-antigen standard or of the samples to be assayed is incubated in the wells of a flexible vinyl Microtiter plate coated with D-antigen specific antibody. The poliovirus D-antigen bound to the antibody coated wells is quantitated by addition of the same antibody radioactively labelled with n-succinimidyl (2, 3-3H) propionate; the D-antigen content of the samples is determined by means of the standard curve generated by the D-antigen standard dilution series. Both the coating antibody and the radioactively labelled antibody may be freeze dried, and this provides a highly stable assay system with a long shelf-life.