Related Experiment Video
Updated: Aug 9, 2026

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
Published on: November 16, 2011
Insulin acting as a modulator of feeding through the hypothalamus
Abstract:
To understand the functional role of insulin in the control of feeding, biochemical and physiological studies were performed in the rat. 1) Insulin content in the brain was much higher than that in the blood, and was extremely variable from animal to animal. 2) Specific binding sites of insulin in the brain were most abundant in the hypothalamus and olfactory bulb. 3) Neither insulin content nor binding sites in the brain were significantly affected by peripheral insulin concentration. 4) Activity of glucoreceptor neurons in the ventromedial hypothalamus (VMH) was facilitated by simultaneous application of insulin and glucose, but inhibited by insulin alone. 5) Activity of the glucose-sensitive neurons in the lateral hypothalamus (LHA) was facilitated by insulin in a dose-dependent manner. 6) Stimulation of the ventral part of the LHA accelerated pancreatic vagal nerve activity. Stimulation of the dorsal part of the LHA and the VMH was inhibitory. 7) Pancreatic splanchnic nerve activity during LHA stimulation tended to show inhibition, but sometimes was modulated by stimulus frequency. Both inhibition and facilitation were observed in the activity in response to VMH stimulation.
More Related Videos
08:47Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
Published on: December 7, 2017
08:32Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain
Published on: January 4, 2018
Related Concept Videos
Hormones Regulating Blood Glucose
In addition to accelerating glucose uptake and utilization, insulin has...
Regulation of Food Intake
Glucose Homeostasis: Regulation of Blood Glucose
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
Insulin and C-peptide are co-secreted in...
Insulin: The Receptor and Signaling Pathways
Glucagon-like Receptor Agonists
GLP-1, when administered in high doses intravenously, triggers insulin secretion, inhibits glucagon release, slows gastric emptying, reduces food intake, and restores normal insulin secretion. However, its rapid inactivation by the...