Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse sperm can horizontally transmit type C viruses.

J A Levy, J Joyner, E Borenfreund

    The Journal of General Virology
    |December 1, 1980
    PubMed
    Summary

    Infectious murine type C viruses (MuLV) were found in AKR and NZB mouse sperm, but not in Balb/c. Sperm can transmit these retroviruses to cells and potentially to offspring, suggesting a role in horizontal MuLV transmission.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cytotoxicity and genotoxicity assays with cultured fish cells: A review.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Cytotoxic and morphological effects of phenylpropanolamine, caffeine, nicotine, and some of their metabolites studied In vitro.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Eugenol cytotoxicity evaluated with continuous cell lines.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Comparisons of two in vitro cytotoxicity assays-The neutral red (NR) and tetrazolium MTT tests.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Structure-activity relationship (SAR) models established in vitro with the neutral red cytotoxicity assay.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    A STUDY OF THE PENETRATION OF MAMMALIAN CELLS BY DEOXYRIBONUCLEIC ACIDS.

    The Journal of biophysical and biochemical cytology·2009

    Area of Science:

    • Virology
    • Reproductive Biology
    • Genetics

    Background:

    • Murine type C viruses (MuLV) are retroviruses known to infect various mouse strains.
    • The potential role of sperm in the transmission of infectious agents, including MuLV, has not been fully elucidated.

    Purpose of the Study:

    • To investigate the presence of infectious MuLV in mouse sperm from different strains.
    • To determine if mouse sperm can transmit infectious MuLV to other cells.
    • To explore potential mechanisms of MuLV transmission via sperm.

    Main Methods:

    • Washed, non-viable and viable sperm from AKR, NZB, Balb/c, and C57Bl/6 mice were used.
    • Sperm were co-cultured with tissue culture cells to assess MuLV transfer.
    • Infectious MuLV was detected using appropriate assays.

    Main Results:

    • Infectious MuLV was detected in non-viable sperm of AKR and NZB mice, but not Balb/c or C57Bl/6.
    • Viable sperm from AKR, NZB, and Balb/c mice transmitted infectious MuLV to tissue culture cells.
    • Virus associated with sperm or proviral DNA are proposed as transmission mechanisms.

    Conclusions:

    • Mouse sperm can harbor and transmit infectious MuLV.
    • Sperm may play a role in the horizontal transmission of MuLV between animals during copulation.
    • Sperm could potentially transmit MuLV to the germ line during fertilization.

    Related Experiment Videos