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Nucleotide sequence changes in polyoma ts-a mutants: correlation with protein structure.

P L Deininger, P LaPorte, T Friedmann

    Journal of Virology
    |March 1, 1981
    PubMed
    Summary
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    Researchers identified specific mutations in polyoma ts-a mutants. These genetic changes occurred in the large T antigen, affecting conserved residues critical for its function.

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Polyoma virus large T antigen plays crucial roles in viral replication and transformation.
    • Understanding mutations in temperature-sensitive (ts) mutants aids in dissecting protein function.

    Purpose of the Study:

    • To determine the precise genetic mutations in three polyoma ts-a mutants.
    • To correlate specific mutations with alterations in the large T antigen.

    Main Methods:

    • DNA sequencing was employed to identify single-base changes in the polyoma ts-a mutants.
    • Mutations were mapped to specific positions within the polyoma virus early region.

    Main Results:

    • Two mutants (ts-25, ts-52) exhibited distinct single-base changes at position 2883, altering a conserved glycine near the C-terminus of large T antigen.

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  • Mutant ts-48 possessed two mutations: one at position 2341 and another at position 1228, affecting regions shared by large and medium T antigens.

    • Conclusions:

    • The identified mutations provide insights into the structure-function relationship of the polyoma large T antigen.
    • These findings contribute to understanding the genetic basis of temperature sensitivity in polyoma virus mutants.