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Related Experiment Videos

Provirus DNA sequences in Rous sarcoma cell genome.

E A Ratovitski, L P Korobitsyn, J D Shaposhnikov

    Neoplasma
    |January 1, 1980
    PubMed
    Summary

    Avian sarcoma virus (ASV) proviral DNA (pro-DNA) organization differs between permissive and non-permissive hosts. Hamster sarcoma pro-DNA contains unique and reiterated sequences, including ASV-specific and endogenous chicken virus (ECV)-specific regions.

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    Area of Science:

    • Molecular Biology
    • Virology
    • Genetics

    Background:

    • Avian sarcoma virus (ASV) is a retrovirus that can integrate into the host genome as proviral DNA (pro-DNA).
    • Understanding the organization of ASV pro-DNA in different host cells is crucial for studying viral replication and pathogenesis.
    • Endogenous chicken virus (ECV)-specific sequences are also present in chicken cells and their interaction with ASV pro-DNA is of interest.

    Purpose of the Study:

    • To investigate the organization and sequence composition of ASV pro-DNA in both permissive and non-permissive host cells.
    • To compare the presence of ASV-specific and ECV-specific nucleotide sequences in hamster and chicken DNA.
    • To elucidate the structural differences in pro-DNA between permissive and non-permissive hosts.

    Main Methods:

    • DNA extraction from non-producer hamster cells, virus-producing chicken Rous sarcoma cells, normal hamster liver, chicken Rous sarcoma cells, chick embryo cells (CEC), and hamster tumor cells.
    • Annealing experiments using ASV-specific and ECV-specific nucleic acid sequences.
    • Analysis of pro-DNA organization, including moderately reiterated and unique sequences.

    Main Results:

    • Complete ASV pro-DNA was found in non-producer hamster and virus-producing chicken cells, but absent in normal hamster liver DNA.
    • Chicken Rous sarcoma and CEC DNA contained nearly all ECV-specific sequences, while hamster tumor DNA contained over half.
    • Hamster sarcoma pro-DNA uniquely contained "sarcoma-specific" and "common" sequences, alongside moderately reiterated and unique sequences.

    Conclusions:

    • Permissive host pro-DNA contains "sarcoma-specific", "common", and "endogenous-specific" nucleotide sequences.
    • "Common" regions are mainly in unique zones, while "sarcoma-specific" and "endogenous-specific" sequences are in reiterated and unique zones.
    • Significant differences exist in pro-DNA organization between permissive and non-permissive hosts, influencing viral integration and expression.

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