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Regulatory interactions between mitochondrial genes: interactions between two mosaic genes.

S Dhawale, D K Hanson, N J Alexander

    Proceedings of the National Academy of Sciences of the United States of America
    |March 1, 1981
    PubMed
    Summary

    A specific intron sequence in Saccharomyces cerevisiae

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    Area of Science:

    • Mitochondrial genetics
    • Molecular biology
    • Yeast genetics

    Background:

    • Mosaic genes like cob and oxi3 in Saccharomyces cerevisiae contain intervening sequences.
    • Understanding the function of these intervening sequences is crucial for gene regulation.
    • Previous studies have hinted at complex regulatory mechanisms within mitochondrial genes.

    Purpose of the Study:

    • To investigate the role of specific intervening sequences in the cob and oxi3 mosaic genes.
    • To elucidate the regulatory relationship between the cob and oxi3 genes.
    • To characterize the BOX phenotype and its genetic basis.

    Main Methods:

    • Analysis of mitochondrial DNA and phenotypes in Saccharomyces cerevisiae strains.
    • Genetic manipulation to study the effects of specific intervening sequences.

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  • Biochemical analysis of polypeptide accumulation in mutant strains.

    • Main Results:

    • A downstream intervening sequence (box7) in the cob gene is sufficient for regulating the oxi3 gene (BOX phenotype).
    • Upstream sequences in cob and oxi3 are dispensable for this regulation.
    • Mutations in the regulatory segment mimic box7 mutants, leading to polypeptide accumulation.

    Conclusions:

    • A hypothetical product from the cob gene's intron (box7) likely controls the expression of the oxi3 gene.
    • The presence of a specific downstream intervening sequence is key for this intergenic regulation.
    • This study provides a model for intron-mediated gene expression control in mitochondria.