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DNA binding of a nonstructural reovirus protein
Canadian Journal of Biochemistry
|February 1, 1981
Summary
Reovirus infection inhibits DNA synthesis by targeting the cell nucleus. Researchers found that the reovirus nonstructural (NS) sigma protein binds to DNA, potentially disrupting chromosome function.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Reovirus infection causes early inhibition of DNA synthesis.
- The cell nucleus is a suspected target for virus-induced damage.
Purpose of the Study:
- To investigate the affinity of reovirus proteins for DNA.
- To determine if DNA binding by viral proteins could explain DNA synthesis inhibition.
Main Methods:
- Chromatography of cytoplasmic and nuclear cell extracts on DNA-cellulose columns.
- Electrophoretic analysis of reovirus-specific proteins using radiolabeling.
- Testing affinity of viral proteins for native and denatured DNA.
Main Results:
- A reovirus nonstructural (NS) sigma protein was identified with high affinity for both native and denatured DNA.
- This DNA-binding protein was found in both cytoplasmic and nuclear extracts.
- Purified reovirus particles showed minimal DNA binding, suggesting the protein is not a surface structural component.
Conclusions:
- The reovirus NS sigma protein exhibits significant DNA-binding properties.
- This DNA-binding capability offers a potential mechanism for reovirus-induced inhibition of host cell DNA synthesis.
- The interaction may interfere with normal chromosome function in infected cells.