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Factors affecting Sertoli cell function in the testis.

D J Tindall, J S Tash, A R Means

    Environmental Health Perspectives
    |April 1, 1981
    PubMed
    Summary
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    Follicle-stimulating hormone (FSH) regulates Sertoli cell protein synthesis by increasing cyclic AMP (cAMP) levels. This leads to the enhanced production of protein kinase inhibitor (PKI) and androgen-binding protein (ABP).

    Area of Science:

    • Reproductive Endocrinology
    • Molecular Cell Biology
    • Biochemistry

    Background:

    • Sertoli cells in the mammalian testis are the primary targets for follicle-stimulating hormone (FSH).
    • FSH receptors on Sertoli cells regulate adenylyl cyclase and cAMP phosphodiesterase activity.
    • FSH influences intracellular cyclic adenosine monophosphate (cAMP) levels, impacting cellular processes.

    Purpose of the Study:

    • To investigate the intracellular mechanisms by which FSH regulates protein synthesis and secretion in Sertoli cells.
    • To identify the initial intracellular proteins affected by FSH and their temporal relationship to cAMP production.
    • To elucidate the role of cAMP in mediating FSH-stimulated protein synthesis and secretion.

    Main Methods:

    • Experiments were conducted both in vivo and in vitro using isolated Sertoli cells.

    Related Experiment Videos

  • Measurements included adenylyl cyclase activity, cAMP phosphodiesterase activity, and intracellular cAMP levels.
  • Analysis of RNA and protein synthesis, specifically focusing on protein kinase inhibitor (PKI) and androgen-binding protein (ABP).

    • Main Results:

    • FSH stimulation rapidly increased intracellular cAMP levels by modulating adenylyl cyclase and cAMP phosphodiesterase.
    • FSH selectively elevated the synthesis of protein kinase inhibitor (PKI) in Sertoli cells, preceding other protein changes.
    • FSH also enhanced the secretion of androgen-binding protein (ABP), with PKI synthesis occurring before ABP secretion.

    Conclusions:

    • FSH primarily acts on Sertoli cells by increasing intracellular cAMP levels.
    • Elevated cAMP mediates the FSH-induced synthesis of PKI and subsequent secretion of ABP.
    • The data support a model where FSH-regulated protein synthesis and secretion are largely controlled by cAMP.