Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spatial resolution and detection sensitivity in microanalysis by electron loss selected imaging.

K M Adamson-Sharpe, F P Ottensmeyer

    Journal of Microscopy
    |June 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Freestanding lipid bilayers as substrates for electron cryomicroscopy of integral membrane proteins.

    Journal of microscopy·2002
    Same author

    Ca(2+) shuttling in vesicles during tip growth in Neurospora crassa.

    Fungal genetics and biology : FG & B·2001
    Same author

    Quaternary organization of the Staphylothermus marinus phosphoenolpyruvate synthase: angular reconstitution from cryoelectron micrographs with molecular modeling.

    Journal of structural biology·2001
    Same author

    Localization of chromophore absorption signals in TEM with an improved prism-mirror-prism filter.

    Journal of electron microscopy·2000
    Same author

    Mechanism of transmembrane signaling: insulin binding and the insulin receptor.

    Biochemistry·2000
    Same author

    Angular reconstitution of the Staphylothermus marinus phosphoenolpyruvate synthase.

    Microscopy research and technique·2000
    Same journal

    In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

    Journal of microscopy·2026
    Same journal

    The future of DXA: How AI is transforming bone health diagnostics.

    Journal of microscopy·2026
    Same journal

    The Origins of Ploem's Filter Cube: A Pandora's Box.

    Journal of microscopy·2026
    Same journal

    The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

    Journal of microscopy·2026
    Same journal

    Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

    Journal of microscopy·2026
    Same journal

    Modular training resources for bioimage analysis.

    Journal of microscopy·2026
    See all related articles

    A new electron energy filter achieved high-resolution imaging of murine leukaemia virus. This technique offers excellent spatial resolution for visualizing virus structures and elemental composition.

    Area of Science:

    • Materials Science
    • Biophysics
    • Analytical Chemistry

    Background:

    • High-resolution transmission electron microscopy (HRTEM) is crucial for nanoscale analysis.
    • Electron energy filters enable elemental mapping and chemical state analysis.
    • Understanding virus structure and composition is vital for virology and disease research.

    Purpose of the Study:

    • To evaluate the sensitivity and spatial resolution of a Castaing-Henry type electron energy filter.
    • To assess the filter's performance in energy-selected imaging of biological samples.
    • To determine the minimum detectable mass and finest structural detail achievable.

    Main Methods:

    • Utilized a Castaing-Henry type electron energy filter coupled with HRTEM.
    • Acquired energy-selected images of murine leukaemia virus at a specific electron energy loss.

    Related Experiment Videos

  • Performed electron spectroscopic imaging to analyze phosphorus distribution within the virus membrane.

    • Main Results:

    • Achieved a best spatial resolution between 0.3 and 0.5 nm for virus imaging.
    • Demonstrated a high sensitivity of 2 x 10(-21) g for elemental detection.
    • Successfully visualized phosphorus within the virus membrane bilayer.

    Conclusions:

    • The Castaing-Henry electron energy filter provides high spatial resolution for nanoscale biological imaging.
    • The filter exhibits excellent sensitivity for detecting trace elements in biological structures.
    • This technology advances the capability for detailed structural and elemental analysis of viruses.