Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Herpes simplex virus (type 1) thymidine kinase gene does not transform cells morphologically.

B Hampar, J G Derge, A L Boyd

    Proceedings of the National Academy of Sciences of the United States of America
    |April 1, 1981
    PubMed
    Summary

    Herpes simplex virus type 1 (HSV-1) can independently cause morphological and biochemical cell transformations. The HSV-1 thymidine kinase (TK) gene is a potential tool for assessing gene transforming potential.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Breast cancer and other neoplasms in women with neurofibromatosis type 1: a retrospective review of cases in the Detroit metropolitan area.

    American journal of medical genetics. Part A·2012
    Same author

    Diverse system stresses: common mechanisms of chromosome fragmentation.

    Cell death & disease·2011
    Same author

    Preparation of high-molecular-weight DNA for use in DNA transfection : secondary transfections for cloning active genes by direct phenotypic selection.

    Methods in molecular biology (Clifton, N.J.)·2011
    Same author

    Critical pathways in cellular senescence and immortalization revealed by gene expression profiling.

    Oncogene·2008
    Same author

    Anagogy of autonomy.

    Eubios journal of Asian and international bioethics : EJAIB·2005
    Same author

    Genetic alteration of chromosome 8 is a common feature of human mammary epithelial cell lines transformed in vitro with benzo[a]pyrene.

    Mutation research·2001

    Area of Science:

    • Virology
    • Molecular Biology
    • Cancer Research

    Background:

    • BALB/c-derived 10E2 cells were rendered thymidine kinase (TK)-negative.
    • A specific clone, B2, was selected for studying transformations induced by herpes simplex virus type 1 (HSV-1).
    • Untransformed B2 cells exhibited a normal morphology and were nontumorigenic in nude mice.

    Purpose of the Study:

    • To investigate the independent roles of morphological and biochemical transformations induced by HSV-1.
    • To evaluate the potential of the HSV-1 thymidine kinase (TK) gene as a vehicle for gene transfer and transformation studies.

    Main Methods:

    • B2 cells were infected with UV-inactivated HSV-1 (UV-HSV) and cultured in normal or TK-selective media.
    • Morphological changes were observed in cells cultured in normal medium.

    Related Experiment Videos

  • Biochemical transformation was assessed by gene transfection and selection for TK expression.
  • Tumorigenicity of transformed cells was tested in nude mice.

    • Main Results:

    • UV-HSV infection in normal medium induced morphological transformation, resulting in spindle-shaped cells that were tumorigenic and TK-negative.
    • UV-HSV infection or HSV-1 TK gene transfection in selective medium led to biochemical transformation, characterized by normal cell morphology, TK expression, and nontumorigenicity.
    • Morphological and biochemical transformations were found to be independent events.

    Conclusions:

    • Herpes simplex virus type 1 (HSV-1) can induce independent morphological and biochemical transformations in cells.
    • The HSV-1 TK gene serves as a suitable marker for biochemical transformation and can be utilized for introducing other genes to assess their transforming potential.