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Transforming growth factor production by chemically transformed cells
Cancer Research
|July 1, 1981
Summary
Chemically transformed mouse cells secrete transforming growth factors (TGFs) that induce anchorage-independent growth in normal cells. These novel TGFs, distinct from previously identified ones, were purified and characterized.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Chemically transformed murine cell lines AKR-MCA and C3H/MCA-58 produce growth factors.
- These factors can induce transformed phenotypes in normal cells.
Purpose of the Study:
- To isolate and characterize the transforming growth factors (TGFs) produced by chemically transformed murine cells.
- To determine if these TGFs are similar to previously identified growth factors.
Main Methods:
- Serum-free conditioned medium from transformed cells was dialyzed, lyophilized, and chromatographed.
- Bio-Gel P-60 and carboxymethylcellulose chromatography were used for separation.
- Indicator cells (AKR-2B, NRK) were used to assay for soft agar growth stimulation and DNA synthesis.
Main Results:
- A growth-stimulating activity was detected in the 10,000-12,000 molecular weight range.
- The activity was trypsin and dithiothreitol sensitive, and heat stable.
- Three peaks of activity were found after further chromatography; major peaks did not compete for epidermal growth factor receptor binding.
Conclusions:
- Chemically transformed murine cells produce novel transforming growth factors.
- These TGFs differ from those found in other transformed cell types.
- The major TGFs identified do not significantly interact with the epidermal growth factor receptor.