Related Experiment Video

Updated: Jul 25, 2026

In Vitro Transcription Assays and Their Application in Drug Discovery
09:28

In Vitro Transcription Assays and Their Application in Drug Discovery

Published on: September 20, 2016

The nus mutations affect transcription termination in Escherichia coli

D F Ward, M E Gottesman

    Nature
    |July 16, 1981
    PubMed

    More Related Videos

    Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach12:12

    Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

    Published on: March 12, 2017

    Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli07:04

    Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

    Published on: February 5, 2019

    Related Concept Videos

    Mismatch Repair01:36

    Mismatch Repair

    Overview
    Nucleotide Excision Repair01:08

    Nucleotide Excision Repair

    Overview
    Nonsense-mediated mRNA Decay02:27

    Nonsense-mediated mRNA Decay

    The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
    Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
    Transcription Attenuation in Prokaryotes02:42

    Transcription Attenuation in Prokaryotes

    Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
    There are several different mechanisms used to attenuate transcription. In ribosome mediated...
    Nucleotide Excision Repair01:38

    Nucleotide Excision Repair

    DNA Distortion and Damage
    Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
    Mismatch Repair01:20

    Mismatch Repair

    Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
    The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
    The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...